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N key education. In each villages, most households raised backyard poultry N primary education. In both villages, most households raised backyard poultry [13]. Their main source of earnings was crop farming. In June-July 2009, the typical quantity of backyard poultry in poultry raising households was 987 in the Rajshahi village (with an average of nine poultry per household) and 993 in title= s00221-011-2677-0 the Chittagong village (with an average of eight poultry per household). Our informants were mostly girls, who mostly carried out poultry-related activities and have been the main caregivers and selection makers for poultry inside that household. A number of our informants were men, who the investigation team observed slaughtering poultry. Sixty two % of informants attended college; 37 were between grade 1 and five, and 25 attended grade 6 and above.Change in awarenessThe research team completed the field notes and transcribed the recorded data verbatim in Bengali. They performed thematic analysis [36] of the field notes and transcripts. Initially, they study the transcriptions and field notes repeatedly to get a sense of title= journal.pcbi.1005422 the information set. 4 members (NAR, KI, MZR and MH) of the analysis team individually came up with lists of codes (e.g., indicators of avian influenza, routes of transmission from poultry to humans, slaughtering sick poultry, disposing poultryMore villagers reported hearing about `bird flu' just after the intervention in comparison to before the intervention (97 versus 29 ) (Table 2). Ahead of the intervention, informants talked about bird flu as a disease of broiler or industrial farm chickens or a foreign illness, which will not happen in Bangladesh. A handful of informants recalled one particular or two signs and reported that sudden death was aRimi et al. BMC Public Wellness (2016) 16:Web page six ofTable 2 Awareness of avian influenza illness and its route of transmission just before and immediately after intervention, Rajshahi and Chittagong villages, Bangladesh,Topics [Intervention messages] Interviews just before intervention (Na = 42) Responses Heard/knew about bird flu illness [Bird flu is often a poultry disease] Interviews soon after intervention (N* = 36) Number of interviews ( ) with responses (97) Quantity of interviews ( ) Responses with responses- A disease of broiler/ 12b farm poultry, not of backyard poultry - Several poultry died/ killed by government - Conspiracy of foreign country - A disease of chickens, not ducks - A birds' disease caused from flu/cold - A foreign disease/did not take place in our village/country - Sudden death - Fever - Gizzard melts and chickens die in 24 h 3b(29) - A disease of poultry/ 35b chicken/backyard chicken, which also can infect humans - A hazardous infectious virus of chickens which can also infect humansSigns in poultry [Poultry die really swiftly in this illness. They get drowsy, and the wattle and comb come to be blackish blue. Wattle, comb, head and physique swells with water. They get blood spots in legs.](7)- Blue/blackish wattle/ 24b comb - Blood/black spots in leg - Drowsiness/sit quiet - Swollen body - Fever - Liquid defecation - Stop consuming - Secret saliva - Cold/coughing 19 - If kept in exact same place 18b with sick poultry - Consuming from very same pot with sick poultry - Scavenging in the feces of sick poultry - Get in touch with with saliva/ feces/blood/offal/ skin/feathers of sick/ dead poultry - Through breathing or air(67)Can transmit from poultry to poultry [Bird flu can spread from one particular poultry to another] Route of transmission from poultry to - Migratory bird poultry [Keeping wholesome and sick poultry inside the similar location; giving food in the exact same plate; pecking on feces of sick pou.